An in vitro human mammary epithelial cell permeability assay to assess drug secretion into breast milk
Determining the amount of a drug transferred into breast milk is critical for benefit-risk analysis of breastfeeding when a lactating mother takes medications.In this study, we developed a human mammary epithelial cell (MEC)-based permeability assay to assess drug permeability across the mammary epithelium.Human MEC cell MCF10F formed tight junctio